Plasmid ID | PID00025 |
Plasmid Name | pMTT1-G1 |
Academic Cost | $60 |
Base Pairs | 7974 |
Source | Jacek Gaertig, University of Georgia |
Publications | |
Vector Type | Vector for targeting MTT1 driven gene to BTU1 locus |
E. coli selection | Ampicillin |
Tetrahymena selection | Taxol- only with strains carrying mutation in Btu1 gene conferring taxol sensitivity |
Selection cassette | Mutation in BTU1 conferring taxol sensitivity |
Expression cassette | |
Notes | can ONLY be used with CU522 and related strains to target BTU1 locus using taxol selection. Additional selection cassette could be added to target other strains |
Sequence File | pMTT1G1.docx |
Plasmid Map |