Plasmid ID | PID00021 |
Plasmid Name | pMNMM3-HA-Cre1 |
Academic Cost | $60 |
Base Pairs | 8342 |
Source | Kazufumi Mochizuki, IMBA, Vienna, Austria |
Publications | Busch CJ, Vogt A, Mochizuki K. 2010. Establishment of a Cre/loxP recombination system for N-terminal epitope tagging of genes in Tetrahymena. BMC Microbiol 10:191. |
Vector Type | Targets HA-Cre1 to MTT1 locus |
E. coli selection | Ampicillin |
Tetrahymena selection | Paromomycin |
Selection cassette | neo5 [HHFI(promoter)-codon optimized neo; constitutive promoter |
Expression cassette | HA-cre1 under inducible MTT1 promoter, targeted to MTT1 locus |
Notes | |
Sequence File | pMNMM3-HA-cre1.docx |
Plasmid Map |