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Plasmid IDPID00013
Plasmid NamepMrpL29b
Academic Cost$50
Base Pairs4674
SourceMarty Gorovsky lab, University of Rochester
Vector TypeCycloheximide selection cassette (rpL29 cDNA)
E. coli selectionAmpicillin
Tetrahymena selectionCycloheximide
Selection cassetteMTT1(promoter)-rpL29cDNA
Expression cassette
NotesPlasmid Bluescript KS+ with MTT5'(930bases) -rpL29-BTU inserted at EcoRI site, lost Hind III site and surrounding bases. Gives cycloheximide resistance. MTT15' 720-1640, rpL29 1641-2090, BTU23' 2091-2440.
Sequence File pM-rpl29B.docx
Plasmid Map