Tetrahymena Stock Center| Plasmid ID | PID00012 |
| Plasmid Name | pHrpl29b |
| Academic Cost | $60 |
| Base Pairs | 4078 |
| Source | Marty Gorovsky lab, University of Rochester |
| Publications | |
| Vector Type | Cycloheximide selection cassette (rpL29 cDNA) |
| E. coli selection | Ampicillin |
| Tetrahymena selection | Cycloheximide |
| Selection cassette | HHFI(promoter)-rpL29cDNA |
| Expression cassette | |
| Notes | Plasmid Bluescript KS+ with 330 bases of HHFI promoter starting at 712, rpL29 starts at 1044, and BTU2 termination region at 1494. Cassette inserted at EcoRI site, lost Hind III site and surrounding bases. Gives cycloheximide resistance |
| Sequence File | pH-rpl29-B.docx |
| Plasmid Map |
