Skip to main content

Follow @TetrahymenaSC

Plasmid IDPID00012
Plasmid NamepHrpl29b
Academic Cost$50
Base Pairs4078
SourceMarty Gorovsky lab, University of Rochester
Vector TypeCycloheximide selection cassette (rpL29 cDNA)
E. coli selectionAmpicillin
Tetrahymena selectionCycloheximide
Selection cassetteHHFI(promoter)-rpL29cDNA
Expression cassette
NotesPlasmid Bluescript KS+ with 330 bases of HHFI promoter starting at 712, rpL29 starts at 1044, and BTU2 termination region at 1494. Cassette inserted at EcoRI site, lost Hind III site and surrounding bases. Gives cycloheximide resistance
Sequence File pH-rpl29-B.docx
Plasmid Map