Tetrahymena Stock Center| Plasmid ID | PID00010 |
| Plasmid Name | pMTT1GOI-GFPBTU1 (pMTT-NRK2-GFP) |
| Academic Cost | $60 |
| Base Pairs | 9774 |
| Source | Jacek Gaertig lab, University of Georgia |
| Publications | |
| Vector Type | Add 3' GFP to GOI. Targets BTU1 locus |
| E. coli selection | Ampicillin |
| Tetrahymena selection | Taxol - only with strains carrying mutation in Btu1 gene conferring taxol sensitivity |
| Selection cassette | Taxol in strains with BTU1 mutation conferring taxol sensitivity |
| Expression cassette | MTT1 (inducible promoter) driven GOI tagged with GFP, targeted to BTU1 locus |
| Notes | can ONLY be used with CU522 and related strains to target BTU1 locus using taxol selection. Additional selection cassette could be added to target other strains. 1. The sequence of the 5' restriction sites is  2. Grow in a methylation minus Ecoli strain |
| Sequence File | pMTT-NRK2-GFP.docx |
| Plasmid Map |
