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Plasmid IDPID00010
Academic Cost$50
Base Pairs9774
SourceJacek Gaertig lab, University of Georgia
Vector TypeAdd 3' GFP to GOI. Targets BTU1 locus
E. coli selectionAmpicillin
Tetrahymena selectionTaxol - only with strains carrying mutation in Btu1 gene conferring taxol sensitivity
Selection cassetteTaxol in strains with BTU1 mutation conferring taxol sensitivity
Expression cassetteMTT1 (inducible promoter) driven GOI tagged with GFP, targeted to BTU1 locus
Notescan ONLY be used with CU522 and related strains to target BTU1 locus using taxol selection. Additional selection cassette could be added to target other strains.

1. The sequence of the 5' restriction sites is

2. Grow in a methylation minus Ecoli strain
Sequence File pMTT-NRK2-GFP.docx
Plasmid Map