Stock ID | SD03308 |
Strain Name | CU428/fsCodopt |
AKA | CU428/crCodopt |
Species | T. thermophila |
Genetic Description | mpr1-1/mpr1-1 (WT; WT, VII) |
MAC Knockins/outs | RPL29::frame shift codon optimized NEO |
MIC Knockins/outs | |
Gene of Interest | |
Origin | CU428 with codon optimized NEO coding sequence with two frameshift mutations. |
Publications | |
Comments | Codon optimized NEO coding sequence with two frameshift mutations in RPL29 vector (conferring resistance to Cyclohexamide, but remaining sensitive to paromomycin SELECT WITH 200ug/mL Pm). The intention is this will provide the OLD MAC with a (defective) copy of the resistance gene, preventing its elimination in the NEW MAC after MIC transformation. From the Winey Lab at the University of Colorado Boulder. |
Research Resource Identifier | TSC_SD03308 |
Requires MTA? | No |
Academic cost | $45 |