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Stock IDSD03298
Strain NameUCB305
AKAsfr13 KO/5'FLAG rescue
SpeciesT. thermophila
Genetic Descriptionsfr13-1[∆::NEO2] (sfr13-1[∆::NEO2]::SFR13[5'FLAG::BSR]::SFR13 3'; WT cell size and number of basal bodies., ?)
MAC Knockins/outsFLAG-SFR13 rescue in two parts (see comments)
MIC Knockins/outscen2-1[∆::NEO2]
Gene of InterestSFR13 (TTHERM_00522830)
OriginUCB301 X UCB302
Commentssfr13 complete null rescue. First a 5' part of the gene was introduced into the sfr13 locus with BSR selection that included a 5'FLAG tag. Second, the remainder of the gene was introduced to the KO locus with the 5' BSR insertion, and rescue of the small cell size phenotype was selected for.

From the Winey Lab at the University of Colorado Boulder.
Research Resource IdentifierTSC_SD03298
Requires MTA?No
Academic cost $35