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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD03073
Strain NameMU137
SpeciesT. thermophila
Genetic Description(pm-r, II)
MAC Knockins/outsASI2 replaced with ASI2Y607F
MIC Knockins/outs
Gene of InterestASI2 (TTHERM_00191480)
OriginSomatic transformant of B2086
CommentsY607 is in a putative SH2 binding site. ASI2 is nonessential for vegetative growth. Matings between ASI2Y607F somatic replacement strains have reduced fertility and delayed endocycling during MAC development.

For paromomycin resistance, add 0.5 ug/ul CdCl2 along with the parmomycin.
Research Resource IdentifierTSC_SD03073
Requires MTA?No
Academic cost $45