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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.


Stock IDSD02964
Strain Name5D-2
AKA
SpeciesT. thermophila
Genetic Descriptionbtu1-?[∆::neo1]/ btu1-?[∆::neo1]; btu2-?[∆::bsr1]/ btu2-?[∆::bsr1]; CHX1/CHX1; mpr1-1/mpr1-1 or MPR1/MPR1 (btu2-?[∆::bsr1]; btu1-?[∆::neo1]/ btu1-? [E437D,E438D,E439D,E440D,E442D]; pm-r)
MAC Knockins/outsBTU1 has only (E437-442 EEEE-E changed to DDDD-D) no WT version; BTU2 is KO.
MIC Knockins/outsknockout of btu1 with neo
knockout of btu2 with bsr
Gene of InterestBTU1 (TTHERM_00348510), BTU2 (TTHERM_00836580)
Originfrom Jacek Gaertig, frozen by Jianming Duan
PublicationsXia L, Hai B, Gao Y, Burnette D, Thazhath R, Duan J, Bre MH, Levilliers N, Gorovsky MA, Gaertig J. 2000. Polyglycylation of tubulin is essential and affects cell motility and division in tetrahymena thermophila. J Cell Biol 149(5):1097-6.

Duan J and Gorovsky MA. 2002. Both carboxy-terminal tails of alpha- and beta-tubulin are essential, but either one will suffice. Curr Biol 12(4):313-6.
CommentsProgeny of DB6B x DB2A, transformed by 5D mutation construct The polyglycylation of beta tubulin is eliminated. Can be rescued by AB1.

From the Gorovsky lab, University of Rochester.
Research Resource IdentifierTSC_SD02964
Requires MTA?No
Academic cost $45