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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02885
Strain NameXS92-5
SpeciesT. thermophila
Genetic Description(MTT1/mtt1[5’neo2, ∆, 2HAn(::HTA.Y 1-122 and 258-388)]; pm-r, ?)
MAC Knockins/outsRegion of HTAY gene encoding AA 1 to 122 and 258 to 388 with two HA tags at n-terminus was inserted into the MTT1 gene.
MIC Knockins/outs
Gene of InterestHTAY (TTHERM_01079200)
OriginXiaoyuan Song
PublicationsNot used in this paper, but about HTAY:

Song X, Bowen J, Miao W, Liu Y, Gorovsky MA. 2012. The nonhistone, N-terminal tail of an essential, chimeric H2A variant regulates mitotic H3-S10 dephosphorylation. Genes Dev 26(6):615-29.
CommentsSomatic transfomation of CU428xB2086 with pXS88. (MTT-neo2-2HA-HTA.Y N122+C131) into MTT1 locus, Incomplete replacement

N122 +C131 means AA1-122 and AA258-388 of HTA.Y are present. Missing the middle section of the coding region.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02885
Requires MTA?No
Academic cost $45