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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02884
Strain NameXS90-14
SpeciesT. thermophila
Genetic Description(MTT1/mtt1[5’neo2, ∆, 2HAn(::HTA.Y 1-258)]; pm-r, ?)
MAC Knockins/outsRegion of HTAY gene encoding AA 1 to 258 with two HA tags at n-terminus was inserted into the MTT1 gene.
MIC Knockins/outs
Gene of InterestHTAY (TTHERM_01079200)
OriginXiaoyuan Song
PublicationsThis construct was not used but this paper is about HTAY:

Song X, Bowen J, Miao W, Liu Y, Gorovsky MA. 2012. The nonhistone, N-terminal tail of an essential, chimeric H2A variant regulates mitotic H3-S10 dephosphorylation. Genes Dev 26(6):615-29.
CommentsSomatic transfomation of CU428xB2086 with pXS88. (MTT-neo2-2HA-HTA.Y n258) into MTT1 locus, Incomplete replacement

n258 means AA 1-258 of HTA.Y are present

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02884
Requires MTA?No
Academic cost $45