Tetrahymena Stock Center| Stock ID | SD02823 |
| Strain Name | pBBN-CDC2 5’ 727-GFP (2) |
| AKA | |
| Species | T. thermophila |
| Genetic Description | btu1-1[K350M]/ btu1-1[K350M] (BTU1 locus replaced by far 5’BTU1 5’--neo3--CDC2 5’ flanking 946bp immediately upstream of ATG--GFP coding--BTU1 3’; pm-r with Cd+2; tax-r, ?) |
| MAC Knockins/outs | BTU1 locus replaced by far 5’BTU1 5’--neo3--CDC2 5’ flanking 946 bp immediately upstream of ATG--GFP coding--BTU1 3’ |
| MIC Knockins/outs | |
| Gene of Interest | CDC2 (TTHERM_01207660) |
| Origin | Xiaoyuan Song |
| Publications | Song X and Gorovsky MA. 2007. Unphosphorylated H1 is enriched in a specific region of the promoter when CDC2 is down-regulated during starvation. Mol Cell Biol 27(5):1925-33. Dou Y, Song X, Liu Y, Gorovsky MA. 2005. The H1 phosphorylation state regulates expression of CDC2 and other genes in response to starvation in tetrahymena thermophila. Mol Cell Biol 25(10):3914-22. |
| Comments | Complete somatic transformant of CU522 cells. Construct was pBBN-CDC2: BTU1 5’(600bp from400-1000bp upstream of BTU1 ATG)--Neo3--CDC2 5’flanking 946 bp immediately upstream of ATG--GFP coding- -1.4 Kb BTU1 3”. BTU1 promoter was deleted. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. |
| Research Resource Identifier | TSC_SD02823 |
| Requires MTA? | No |
| Academic cost | $45 |
