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Stock IDSD02822
Strain NamepBBN-CDC2 5’ 1.6-GFP (8)
AKA
SpeciesT. thermophila
Genetic Descriptionbtu1-1[K350M]/ btu1-1[K350M] (BTU1 locus replaced by far 5’BTU1 5’--neo3--CDC2 5’ flanking 1.6kb immediately upstream of ATG--GFP coding--BTU1 3’; pm-r with Cd+2; tax-r, ?)
MAC Knockins/outsBTU1 locus replaced by far 5’BTU1 5’--neo3--CDC2 5’ flanking 1.5 kb immediately upstream of ATG--GFP coding--BTU1 3’
MIC Knockins/outs
Gene of InterestCDC2 (TTHERM_01207660)
OriginXiaoyuan Song
PublicationsSong X and Gorovsky MA. 2007. Unphosphorylated H1 is enriched in a specific region of the promoter when CDC2 is down-regulated during starvation. Mol Cell Biol 27(5):1925-33.

Dou Y, Song X, Liu Y, Gorovsky MA. 2005. The H1 phosphorylation state regulates expression of CDC2 and other genes in response to starvation in tetrahymena thermophila. Mol Cell Biol 25(10):3914-22.
CommentsComplete somatic transformant of CU522 cells. Construct was BTU1 5’(600bp from400-1000bp upstream of BTU1 ATG)--Neo3--CDC2 5’flanking 1600 bp immediately upstream of ATG--GFP coding- -1.4 Kb BTU1 3”. BTU1 promoter was deleted.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02822
Requires MTA?No
Academic cost $35