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Stock IDSD02821
Strain NameMTT-HA-PP50 #9
AKA
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (MTT1/mtt1-?[5’neo2, ∆, HAHAn(::PPO1)]; pm-r, VII)
MAC Knockins/outsPPO1 gene with 2 HA tags at n-terminus partially replace MTT1 coding, also neo2 in 5’ flanking region of MTT1.
MIC Knockins/outs
Gene of InterestPPO1 (TTHERM_00865290, TTHERM_00865300)
OriginJody Bowen
PublicationsSong X, Bowen J, Miao W, Liu Y, Gorovsky MA. 2012. The nonhistone, N-terminal tail of an essential, chimeric H2A variant regulates mitotic H3-S10 dephosphorylation. Genes Dev 26(6):615-29.
CommentsUsed Xiayuan Song’s pXS 75 construct: neo 2 in 5 flank of MTT1 gene. 2 HA tags right after ATG. then PP50 coding sequence. PP50 is Ttherm. 00865300 into Ttherm 00865290 sequence. Need 100ug/ml pm in medium to keep the tagged gene present.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02821
Requires MTA?No
Academic cost $35