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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.


Stock IDSD02775
Strain NameE5 (f3)
AKA
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (hho1[3’neo2, T35E, S43E, S45E, T47E, T54E]; pm-r, VII)
MAC Knockins/outsChange Threonine 35,47,54 and serine 43,45 to glutamic acid
MIC Knockins/outs
Gene of InterestHHO1 (TTHERM_00823720)
OriginNew transformation,done by Wei Miao same plasmid used as original by Yali Dou
PublicationsDou Y, Mizzen CA, Abrams M, Allis CD, Gorovsky MA. 1999. Phosphorylation of linker histone H1 regulates gene expression in vivo by mimicking H1 removal. Mol Cell 4(4):641-7.

Dou Y and Gorovsky MA. 2000. Phosphorylation of linker histone H1 regulates gene expression in vivo by creating a charge patch. Mol Cell 6(2):225-31.
CommentsComplete somatic tranformant of CU428 cells with pHHOA5 /neo construct. Phosphorylation sites 1- 5 of H1 changed to glutamic adid, neo cassette in 3’ flanking region for selection.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02775
Requires MTA?No
Academic cost $45