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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.


Stock IDSD02753
Strain NameB1KO-4
AKA
SpeciesT. thermophila
Genetic DescriptionBTU1/btu1[∆,::H4-neo]; MPR1-1/mpr1-1
MAC Knockins/outs
MIC Knockins/outsH4-neo replaces coding of BTU1, heterozygous
Gene of InterestBTU1 (TTHERM_00348510)
OriginBing Hai
PublicationsHai B and Gorovsky MA. 1997. Germ-line knockout heterokaryons of an essential alpha-tubulin gene enable high-frequency gene replacement and a test of gene transfer from somatic to germ-line nuclei in tetrahymena thermophila. Proc Natl Acad Sci U S A 94(4):1310-5.

Xia L, Hai B, Gao Y, Burnette D, Thazhath R, Duan J, Bre MH, Levilliers N, Gorovsky MA, Gaertig J. 2000. Polyglycylation of tubulin is essential and affects cell motility and division in tetrahymena thermophila. J Cell Biol 149(5):1097-6.
CommentsHeterozygous germline transformant of BTUI knockout. Derived from Cu428 X B2086 transformed w/ BHAB-1(construct). When crossed to CU427, gave cyr and pmr progeny.
Can mate to BIKO-2.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02753
Requires MTA?No
Academic cost $45