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Stock IDSD02751
Strain NameA449-2
AKA
SpeciesT. thermophila
Genetic Descriptionatu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1[Y449A]; pm-r, mp-?)
MAC Knockins/outsATU1 with Y449A, assorted to pm-s
MIC Knockins/outsneo replaces ATU1 coding
Gene of InterestATU1 (TTHERM_00558620)
OriginBing Hai
PublicationsHai B and Gorovsky MA. 1997. Germ-line knockout heterokaryons of an essential alpha-tubulin gene enable high-frequency gene replacement and a test of gene transfer from somatic to germ-line nuclei in tetrahymena thermophila. Proc Natl Acad Sci U S A 94(4):1310-5.

Xia L, Hai B, Gao Y, Burnette D, Thazhath R, Duan J, Bre MH, Levilliers N, Gorovsky MA, Gaertig J. 2000. Polyglycylation of tubulin is essential and affects cell motility and division in tetrahymena thermophila. J Cell Biol 149(5):1097-6.
CommentsIt is a progeny of conjugants of AAK02.7 X AAKO5.5 transformed with pA449 derived from pTUB100E3-PvuII (linearized) where the ATU1 gene is marked with Hae III and Pvu II sites and Y449A.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02751
Requires MTA?No
Academic cost $35