Stock ID | SD02733 |
Strain Name | V5-PP50, M8-A4 |
AKA | |
Species | T. thermophila |
Genetic Description | mpr1-1/mpr1-1 (HA tagged HTAY and V5 tagged PPO1; cy-r, VII) |
MAC Knockins/outs | HA tagged HTAY and PPO1 with n-terminal V5 tag |
MIC Knockins/outs | |
Gene of Interest | PPO1 (TTHERM_00865290) |
Origin | |
Publications | Song X, Bowen J, Miao W, Liu Y, Gorovsky MA. 2012. The nonhistone, N-terminal tail of an essential, chimeric H2A variant regulates mitotic H3-S10 dephosphorylation. Genes Dev 26(6):615-29. |
Comments | Xiaoyuan’s HA2-HTAY cells transformed with V5-PP50 with the cycloheximide resistance cassette H4-rpL29-B upstream. Partial replacement . Cells are very sick, give yellow-green auto fluorescence with many vacuoles. Do give the correct ~36 kDa Western band for anti V5. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. |
Research Resource Identifier | TSC_SD02733 |
Requires MTA? | No |
Academic cost | $45 |