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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02720
Strain NameS-MTT-H3.3-G-32
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (MTT1?/mtt1[5ā€™neo2, āˆ†, (::HHT3,GFPc)]; pm-r with Cd, VII)
MAC Knockins/outsHHT3 with GFP c-terminal tag replaces the MTT1 coding region
MIC Knockins/outs
Gene of InterestHHT3 (TTHERM_00016170)
PublicationsCui B, Liu Y, Gorovsky MA. 2006. Deposition and function of histone H3 variants in tetrahymena thermophila. Mol Cell Biol 26(20):7719-30.
Commentssomatic replace MTT1 coding with H3.3+GFP, neo 2 cassette in 5ā€™ flank. Not sure if complete replacement.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02720
Requires MTA?No
Academic cost $45