Skip to main content


Follow @TetrahymenaSC

We have resumed shipping live cells and will soon ship plasmids and extracted DNA. Transformation services will take longer to resume.


Stock IDSD02720
Strain NameS-MTT-H3.3-G-32
AKA
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (MTT1?/mtt1[5’neo2, ∆, (::HHT3,GFPc)]; pm-r with Cd, VII)
MAC Knockins/outsHHT3 with GFP c-terminal tag replaces the MTT1 coding region
MIC Knockins/outs
Gene of InterestHHT3 (TTHERM_00016170)
Origin
PublicationsCui B, Liu Y, Gorovsky MA. 2006. Deposition and function of histone H3 variants in tetrahymena thermophila. Mol Cell Biol 26(20):7719-30.
Commentssomatic replace MTT1 coding with H3.3+GFP, neo 2 cassette in 5’ flank. Not sure if complete replacement.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02720
Requires MTA?No
Academic cost $45