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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02719
Strain NameS-MTT-H3.2-G-2n5
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (MTT1?/mtt1[5’neo2, ∆, (::HHT2,GFPc)]; pm-r, VII)
MAC Knockins/outsHHT2 with GFP c-terminal tag replaces the MTT1 coding region
MIC Knockins/outs
Gene of InterestHHT2 (TTHERM_00189180)
PublicationsCui B, Liu Y, Gorovsky MA. 2006. Deposition and function of histone H3 variants in tetrahymena thermophila. Mol Cell Biol 26(20):7719-30.
Commentssomatic replace MTT1 coding with H3.2+GFP, neo 2 cassette in 5’ flank. Not sure if complete replacement.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02719
Requires MTA?No
Academic cost $45