Tetrahymena Stock Center| Stock ID | SD02707 |
| Strain Name | ∆P1,4,5 |
| AKA | |
| Species | T. thermophila |
| Genetic Description | mpr1-1/mpr1-1 (hho1[3’neo2,T35A,T47A,T53A]; pm-r, VII) |
| MAC Knockins/outs | Change Threonine 35, 47, and 54 to alanines. |
| MIC Knockins/outs | |
| Gene of Interest | HHO1 (TTHERM_00823720) |
| Origin | |
| Publications | Mizzen CA, Dou Y, Liu Y, Cook RG, Gorovsky MA,Allis CD,. 1999. Identification and mutation of phosphorylation sites in a linker histone. phosphorylation of macronuclear H1 is not essential for viability in tetrahymena. The Journal of Biological Chemistry 274(21):14533-6. Dou Y, Mizzen CA, Abrams M, Allis CD, Gorovsky MA. 1999. Phosphorylation of linker histone H1 regulates gene expression in vivo by mimicking H1 removal. Mol Cell 4(4):641-7. |
| Comments | Complete somatic tranformant of CU428 cells with HHO ∆ P1,4,5 /neo construct. Phosphorylation sites 1 ,4,and 5 of H1 changed to alanine, neo cassette in 3’flanking region for selection. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. |
| Research Resource Identifier | TSC_SD02707 |
| Requires MTA? | No |
| Academic cost | $45 |
