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Stock IDSD02707
Strain Name∆P1,4,5
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (hho1[3’neo2,T35A,T47A,T53A]; pm-r, VII)
MAC Knockins/outsChange Threonine 35, 47, and 54 to alanines.
MIC Knockins/outs
Gene of InterestHHO1 (TTHERM_00823720)
PublicationsMizzen CA, Dou Y, Liu Y, Cook RG, Gorovsky MA,Allis CD,. 1999. Identification and mutation of phosphorylation sites in a linker histone. phosphorylation of macronuclear H1 is not essential for viability in tetrahymena. The Journal of Biological Chemistry 274(21):14533-6.

Dou Y, Mizzen CA, Abrams M, Allis CD, Gorovsky MA. 1999. Phosphorylation of linker histone H1 regulates gene expression in vivo by mimicking H1 removal. Mol Cell 4(4):641-7.
CommentsComplete somatic tranformant of CU428 cells with HHO ∆ P1,4,5 /neo construct. Phosphorylation sites 1 ,4,and 5 of H1 changed to alanine, neo cassette in 3’flanking region for selection.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02707
Requires MTA?No
Academic cost $45