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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02703
Strain NameNNN-12-G
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (ngoA[5’neo3, ∆::ngoA promoter 104bp,GFP]; VII)
MAC Knockins/outsGFP into NgoA locus with 104 bp of NgoA promoter. and neo3 upstream
MIC Knockins/outs
Gene of InterestngoA (TTHERM_00655890)
PublicationsDou Y, Song X, Liu Y, Gorovsky MA. 2005. The H1 phosphorylation state regulates expression of CDC2 and other genes in response to starvation in tetrahymena thermophila. Mol Cell Biol 25(10):3914-22
CommentsXS 35 ngoA 5’-neo3-ngoA ch2p12-GFP-ngoA3’ Somatic transformant replacing WT ngoA gene with GFP coding driven by ngoA 5’ flanking 104 bp. Neo3 cassette is upstream of the 104 bp.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02703
Requires MTA?No
Academic cost $45