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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02700
Strain NameMTT-3HA-HTAY-MTT (XS75-2-2)
SpeciesT. thermophila
Genetic Description(MTT1/mtt1[5’neo2, ∆, HTAY 123-388, 3HAn]; ?)
MAC Knockins/outs3HA n-terminal tagged HTAY gene fragment coding for AA123 to 388into MTT1 locus
MIC Knockins/outs
Gene of InterestHTAY (TTHERM_01079200)
PublicationsSong X, Bowen J, Miao W, Liu Y, Gorovsky MA. 2012. The nonhistone, N-terminal tail of an essential, chimeric H2A variant regulates mitotic H3-S10 dephosphorylation. Genes Dev 26(6):615-29.
Comments(XS 75-2-2) Somatic transformant obtained by shooting CU428 X B2086 exconjugant cells. HTA.Y fused with 3HA tag was used to replace MTT1 coding. Neo2 cassette is the selectable marker. Not completely replaced.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02700
Requires MTA?No
Academic cost $45