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Stock IDSD02692
Strain Namehy3G
SpeciesT. thermophila
Genetic Descriptionhhf1[∆::neo1]/hhf1[∆::neo1]; hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; (Chimeric H3 chimera protein hy3, H3(1-75) H3.3(76-90), H3(91-135); pm-r, ?)
MAC Knockins/outsGFP tagged chimeric H3
MIC Knockins/outsNeo KO of all major HHT genes and HHF genes
Gene of InterestHHF1 (TTHERM_00498190), HHF2 (TTHERM_00189170), HHT1 (TTHERM_00570560), HHT2(TTHERM_00189180)
PublicationsCui B, Liu Y, Gorovsky MA. 2006. Deposition and function of histone H3 variants in tetrahymena thermophila. Mol Cell Biol 26(20):7719-30.
CommentsGermline heteokaryon Major H3 and H4 genes knocked out with neo cassette.It GFP tagged H3 chimera protein hy3, H3(1-75) H3.3(76-90), H3(91-135) rescue the progeny of major H3 H4 KO heterokaryons. can be mated with B2086.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02692
Requires MTA?No
Academic cost $35