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We have resumed shipping live cells and will soon ship plasmids and extracted DNA. Transformation services will take longer to resume.


Stock IDSD02669
Strain Name∆∆HHT3HHT2
AKA
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (HHT2/hht2[∆::neo2]; hht3[∆::neo2]; pm-r, VII)
MAC Knockins/outsNeo2 cassettes KO HHT3, partial KO of HHT2
MIC Knockins/outs
Gene of InterestHHT3 (TTHERM_00016170), HHT4 (TTHERM_00016200)
Origin
PublicationsYu L and Gorovsky MA. 1997. Constitutive expression, not a particular primary sequence, is the important feature of the H3 replacement variant hv2 in tetrahymena thermophila. Mol Cell Biol 17(11):6303-10.

Cui B, Liu Y, Gorovsky MA. 2006. Deposition and function of histone H3 variants in tetrahymena thermophila. Mol Cell Biol 26(20):7719-30.
CommentsHHT2 and HHT3 double knockout, neo 2 cassette replaces both genes

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02669
Requires MTA?No
Academic cost $45