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We have resumed shipping live cells and will soon ship plasmids and extracted DNA. Transformation services will take longer to resume.


Stock IDSD02647
Strain NameHA-hv2 (8)
AKA
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (?HHT3/hht3[3’neo2, HAn]; pm-r, VII)
MAC Knockins/outsN-terminal HA tag of HHT3, not sure if complete replacement
MIC Knockins/outs
Gene of InterestHHT3 (TTHERM_00016170)
Origin
PublicationsYu L and Gorovsky MA. 1997. Constitutive expression, not a particular primary sequence, is the important feature of the H3 replacement variant hv2 in tetrahymena thermophila. Mol Cell Biol 17(11):6303-10.

Cui B, Liu Y, Gorovsky MA. 2006. Deposition and function of histone H3 variants in tetrahymena thermophila. Mol Cell Biol 26(20):7719-30.
CommentsHA-tagged hv2 transformant. Checked by western blot and immunofluorescent staining

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02647
Requires MTA?No
Academic cost $45