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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.


Stock IDSD02643
Strain NameHA-His-hv2(5)
AKA
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (?HHT3/hht3[3’neo2, HA-His-n]; pm-r, VII)
MAC Knockins/outsN-terminal HA-His- tag of HHT3, not sure if complete replacement
MIC Knockins/outs
Gene of InterestHHT3 (TTHERM_00016170)
Origin
PublicationsYu L and Gorovsky MA. 1997. Constitutive expression, not a particular primary sequence, is the important feature of the H3 replacement variant hv2 in tetrahymena thermophila. Mol Cell Biol 17(11):6303-10.

Cui B, Liu Y, Gorovsky MA. 2006. Deposition and function of histone H3 variants in tetrahymena thermophila. Mol Cell Biol 26(20):7719-30.
CommentsHA-His-tagged hv2 transformant. Checked by western blot. HA-His-hv2 is expressed.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02643
Requires MTA?No
Academic cost $45