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Stock IDSD02639
Strain NameH3.3R
AKA
SpeciesT. thermophila
Genetic Descriptionhhf1[∆::neo1]/hhf1[∆::neo1]; hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; (HHF2HHT.3; pm-r, ?)
MAC Knockins/outsGFP H3.3 with wt HHF2 into HHT2HHF2 loccus
MIC Knockins/outsNeo KO of all major HHT genes and HHF genes
Gene of InterestHHF1 (TTHERM_00498190), HHF2 (TTHERM_00189170), HHT2 (TTHERM_00189180)
Origin
PublicationsCui B, Liu Y, Gorovsky MA. 2006. Deposition and function of histone H3 variants in tetrahymena thermophila. Mol Cell Biol 26(20):7719-30.
CommentsGermline heteokaryon Major H3 and H4 genes knocked out with neo cassette.HHT.3 inyo HHT2 locus with HHF2. HHT3 wt is still there. can be mated with B2086.

From the Gorovsky lab, University of Rochester.
Research Resource IdentifierTSC_SD02639
Requires MTA?No
Academic cost $35