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Stock IDSD02599
Strain NameDRH1g∆∆ D3-11F
AKA
SpeciesT. thermophila
Genetic Descriptiondrh1[∆?::neo4]/drh1[∆?::neo4] (drh1[∆?::neo4]/drh1[∆?::neo4]; pm-r, ?)
MAC Knockins/outsneo4 at 5’ end of coding, some coding remains
MIC Knockins/outsneo4 at 5’end of coding, some coding remains
Gene of InterestDRH1 (TTHERM_00190830)
Origin
Publications
CommentsHomozygous homokaryon KO of DRH1 with neo4 cassette. Mates with DRH1g∆∆ C10-11F
Gene is Dicer Related Helicase, sequence is fromTGD 69.m00174. Mating cells stop at mic selection after MeiosisII Gene is expressed at low level in starve and log, peak expression at 4 hr of conjugation. Somatic KO is complete, when mated they arrest after Meiosis II. Homozygous homokaryon KOs do not grow well.

Needs Cd for pm resistance.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02599
Requires MTA?No
Academic cost $35