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Stock IDSD02598
Strain NameDRH1g∆∆ C10-11F
AKA
SpeciesT. thermophila
Genetic Descriptiondrh1[∆?::neo4]/drh1[∆?::neo4] (drh1[∆?::neo4 ] Neo in 5’end of gene,some coding remains; pm-r, ?)
MAC Knockins/outsneo4
MIC Knockins/outsneo4
Gene of InterestDRH1 (TTHERM_00190830)
Origin
Publications
CommentsHomozygous homokaryon KO of DRH1 with neo4 cassette. Mates with DRH1g∆∆ D3-11F.
Gene is Dicer Related Helicase, sequence is fromTGD 69.m00174. Mating cells stop at mic selection after MeiosisII Homozygous homokaryon. Gene is expressed at low level in starve and log, peak expression at 4 hr of conjugation. Somatic KO is complete, when mated they arrest after Meiosis II. Homozygous homokaryon KOs do not grow well.

Needs Cd for pm resistance.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02598
Requires MTA?No
Academic cost $35