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Stock IDSD02597
Strain NameDRH1 s ∆ C8-10C
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (drh1[∆?::neo3]; pm-r, VII)
MAC Knockins/outsNeo3 cassette replaces part of 5’ end of coding
MIC Knockins/outs
Gene of InterestDRH1 (TTHERM_00190830)
CommentsSomatic KO of DRH1 with neo3 KO2 construct. 5’ end of coding removed. Looks like complete replacement by PCR and when grown w/o selection. Resistant to ~700ug/ml pm. Gene is expressed at low level in starve and log, peak expression at 4 hr of conjugation. Somatic KO is complete, when mated they arrest after Meiosis II. Homozygous homokaryons do not grow well.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02597
Requires MTA?No
Academic cost $45