Tetrahymena Stock Center| Stock ID | SD02596 |
| Strain Name | DRH1 s ∆ C8-10B |
| AKA | |
| Species | T. thermophila |
| Genetic Description | mpr1-1/mpr1-1 (drh1[∆?::neo3]; pm-r, VII) |
| MAC Knockins/outs | Neo3 cassette replaces part of 5’ end of coding |
| MIC Knockins/outs | |
| Gene of Interest | DRH1 (TTHERM_00190830) |
| Origin | |
| Publications | |
| Comments | Somatic KO of DRH1 with neo3 KO2 construct. 5’ end of coding removed. Looks like complete replacement by PCR and when grown w/o selection. Resistant to ~700ug/ml pm. Gene is expressed at low level in starve and log, peak expression at 4 hr of conjugation. Somatic KO is complete, when mated they arrest after Meiosis II. Homozygous homokaryons do not grow well. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. |
| Research Resource Identifier | TSC_SD02596 |
| Requires MTA? | No |
| Academic cost | $45 |
