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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02591
Strain Name∆DCLI(s)HAT11(1-1)
SpeciesT. thermophila
Genetic Descriptiondcl1[∆?::neo3]/dcl1[∆?::neo3] (Complete KO of DCL1 with neo3; ?)
MAC Knockins/outsNeo3 KO of DCL1
MIC Knockins/outsHA tagged TWI 11
Gene of InterestDCL1 (TTHERM_00284230), TWI11 (TTHERM_00144830)
PublicationsMochizuki K and Gorovsky MA. 2005. A dicer-like protein in tetrahymena has distinct functions in genome rearrangement, chromosome segregation, and meiotic prophase. Genes Dev 19(1):77-89.
CommentsComplete somatic DCL1 knockout with HA-tagged Twi11 in the Mic, to study Twi11 localization and loading with scnRNA.

Mates with ∆DCL1(s)HAT11(5-1).

From the Gorovsky lab, University of Rochester.
Research Resource IdentifierTSC_SD02591
Requires MTA?No
Academic cost $45