Stock ID | SD02579 |
Strain Name | ch3G |
AKA | |
Species | T. thermophila |
Genetic Description | hhf1[∆::neo1]/hhf1[∆::neo1]; hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; (Chimeric H3 with GFP tagged H3 chimera protein 3, H3(1-75) H3.3(76-135); pm-r, ?) |
MAC Knockins/outs | GFP tagged chimeric H3 |
MIC Knockins/outs | Neo KO of all major HHT genes and HHF genes |
Gene of Interest | HHF1 (TTHERM_00498190), HHF2 (TTHERM_00189170), HHT1 (TTHERM_00570560), HHT2 (TTHERM_00189180) |
Origin | |
Publications | Cui B, Liu Y, Gorovsky MA. 2006. Deposition and function of histone H3 variants in tetrahymena thermophila. Mol Cell Biol 26(20):7719-30 |
Comments | Germline heteokaryon Major H3 and H4 genes knocked out with neo cassette.It GFP tagged H3 chimera protein 3, H3(1-75) H3.3(76-135), rescue the progeny of major H3 H4 KO heterokaryons. can be mated with B2086. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. |
Research Resource Identifier | TSC_SD02579 |
Requires MTA? | No |
Academic cost | $45 |