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Stock IDSD02578
Strain NameCDC5’-GFP-CDC3’-neo3-∆H1 de(P) (XS68)
AKA
SpeciesT. thermophila
Genetic Description? (Partial replacement CDC2/cdc2-? CDC2 gene 5’ flanking sequence of dephosphorylated HI enriched region and coding removed and replaced with GFP gene. Also neo3 in 3’ flanking.; pm-r with Cd+2)
MAC Knockins/outsCDC2 gene 5’ flanking sequence of dephosphorylated HI enriched region and coding removed and replaced with GFP gene. Also neo3 in 3’ flanking.
MIC Knockins/outs
Gene of InterestCDC2 (TTHERM_01207660)
Origin
PublicationsSong X and Gorovsky MA. 2007. Unphosphorylated H1 is enriched in a specific region of the promoter when CDC2 is down-regulated during starvation. Mol Cell Biol 27(5):1925-33.

Dou Y, Song X, Liu Y, Gorovsky MA. 2005. The H1 phosphorylation state regulates expression of CDC2 and other genes in response to starvation in tetrahymena thermophila. Mol Cell Biol 25(10):3914-22.
CommentsXS68 Somatic transformant obtained by shooting CU428 X B2086 exconjugant cells.WT gene CDC2 locus was partially replaced with GFP coding driven by CDC2 5’ flanking region that has deleted H1-dephosphorylated enriched region,. Neo3 is in the CDC2 3’ flanking region

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02578
Requires MTA?No
Academic cost $35