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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02577
Strain NameCDC5’ 727-GFP-CDC3’-neo3 (XS69)
SpeciesT. thermophila
Genetic Description? (Partial replacement CDC2/cdc2-? CDC2 gene 5’ flanking sequence of 942 bp with GFP coding replacing CDC2 coding. Also neo 3 in 3’ flanking.; pm-r with Cd+2, ?)
MAC Knockins/outsCDC2 gene has the 942 CDC2 5’ flanking region that includes the H1 dephosphorylation enriched region and GFP coding replacing CDC2 coding. Also neo3 in 3’ flanking. region.
MIC Knockins/outs
Gene of InterestCDC2
PublicationsSong X and Gorovsky MA. 2007. Unphosphorylated H1 is enriched in a specific region of the promoter when CDC2 is down-regulated during starvation. Mol Cell Biol 27(5):1925-33.

Dou Y, Song X, Liu Y, Gorovsky MA. 2005. The H1 phosphorylation state regulates expression of CDC2 and other genes in response to starvation in tetrahymena thermophila. Mol Cell Biol 25(10):3914-22.
CommentsXS72 Somatic transformant obtained by shooting CU428 X B2086 exconjugant cells. In CDC2 locus, coding region replaced by GFP coding. Shortened CDC2 5’ flanking region is 942 bp that includes the H1dephosphorylation enriched region . Neo3 cassette is in 3’ flanking region.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02577
Requires MTA?No
Academic cost $45