Skip to main content

Follow @TetrahymenaSC

Stock IDSD02573
Strain NameCDC5’ 0-GFP-CDC3’-neo3 (XS73)
SpeciesT. thermophila
Genetic Description? (Partial replacement CDC2/cdc2-? CDC2 gene 5’ flanking sequence of 0 bp with GFP coding replacing CDC2 coding. Also neo3 in 3’ flanking.; pm-r with Cd+2, ?)
MAC Knockins/outsCDC2 gene has the 1.6 kb just upstream of ATG lacking and GFP coding replacing CDC2 coding. Also neo3 in 3’ flanking. region.
MIC Knockins/outs
Gene of InterestCDC2 (TTHERM_01207660)
PublicationsSong X and Gorovsky MA. 2007. Unphosphorylated H1 is enriched in a specific region of the promoter when CDC2 is down-regulated during starvation. Mol Cell Biol 27(5):1925-33.

Dou Y, Song X, Liu Y, Gorovsky MA. 2005. The H1 phosphorylation state regulates expression of CDC2 and other genes in response to starvation in tetrahymena thermophila. Mol Cell Biol 25(10):3914-22.
CommentsXS72 Somatic transformant obtained by shooting CU428 X B2086 exconjugant cells. In CDC2 locus, coding region replaced by GFP coding. Shortened CDC2 5’ flanking region is 0 bases but the CDC2 far 5’ flanking 1 kb sequence that is 1.6 b upstream of the ATG was used to do the homologous recombination. Neo3 cassette is in 3’ flanking region.

From the Gorovsky lab, University of Rochester.
Research Resource IdentifierTSC_SD02573
Requires MTA?No
Academic cost $45