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Stock IDSD02572
Strain NameCDC2-GFP-CDC3’-neo3 (XS67)
AKA
SpeciesT. thermophila
Genetic Description? (Partial replacement CDC2/cdc2-? [3’ neo3, ∆,(::GFP)]; pm-r with Cd+2, ?)
MAC Knockins/outsCDC2 gene has the 942 CDC2 5’ flanking region that includes the H1 dephosphorylation enriched region and GFP coding replacing CDC2 coding. Also neo3 in 3’ flanking. region.
MIC Knockins/outs
Gene of InterestCDC2 (TTHERM_01207660)
Origin
PublicationsSong X and Gorovsky MA. 2007. Unphosphorylated H1 is enriched in a specific region of the promoter when CDC2 is down-regulated during starvation. Mol Cell Biol 27(5):1925-33.

Dou Y, Song X, Liu Y, Gorovsky MA. 2005. The H1 phosphorylation state regulates expression of CDC2 and other genes in response to starvation in tetrahymena thermophila. Mol Cell Biol 25(10):3914-22.
CommentsXS67 Somatic transformant obtained by shooting CU428 X B2086 exconjugant cells. In CDC2 locus, coding region replaced by GFP coding. Neo3 cassette is in 3’ flanking region.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02572
Requires MTA?No
Academic cost $35