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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02547
Strain NameWT H4II/H3II-F
SpeciesT. thermophila
Genetic Descriptionhht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆,::neo2]/hhf2,hht2[∆,::neo2] (hht1[∆::neo2]; pm-r, ?)
MAC Knockins/outsHHF1 ko and HHF2/HHT2 is now WT
MIC Knockins/outsKO of HHT1,HHT2,HHF2
Gene of InterestHHT2 (TTHERM_00189180)
PublicationsWei Y, Yu L, Bowen J, Gorovsky MA, Allis CD. 1999. Phosphorylation of histone H3 is required for proper chromosome condensation and segregation. Cell 97(1):99-109.
CommentsMate Yifan Liu’s ∆H3 2-6 x ∆H3 10-6 homozygous heterokaryons (∆HHT1 with neo, and ∆HHT2/HHF2 with neo in mic) assorted to Paromomycin sensitive in mac. Shoot with Yifan’s wildtype HHT2/HHF2 construct.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02547
Requires MTA?No
Academic cost $45