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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02545
Strain NameWT H4II/H3II-6
SpeciesT. thermophila
Genetic Descriptionhht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆,::neo2]/hhf2,hht2[∆,::neo2] (Neo 2 KO of HHT1, WT HHT2/HHF2; pm-r, ?)
MAC Knockins/outsNeo 2 Ko of HHT1, WT HHT2/HHF2
MIC Knockins/outsNeo 2 KO of HHT1 and of HHT2/HHF2
Gene of InterestHHT1 (TTHERM_00570560), HHT2 (TTHERM_00189180)
PublicationsLiu Y. 2003. Genetic studies of RNA-mediated DNA elimination in the developing macronuclei of tetrahymena thermophila. University of Rochester.
CommentsMate Yifan Liu’s ∆H3 2-6 x ∆H3 10-6 homozygous heterokaryons (∆HHT1 with neo, and ∆HHT2/HHF2 with neo in mic) assorted to Paromomycin sensitive in mac. Shoot with Yifan’s wildtype HHT2/HHF2 construct.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02545
Requires MTA?No
Academic cost $45