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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02539
Strain NameV5-PP50, B4-F5
SpeciesT. thermophila
Genetic Description(cy-r, II)
MAC Knockins/outsPpo1p with n-terminal V5 tag, cells very sick, not used in paper
MIC Knockins/outs
Gene of InterestPPO1 (TTHERM_00865290)
PublicationsSong X, Bowen J, Miao W, Liu Y, Gorovsky MA. 2012. The nonhistone, N-terminal tail of an essential, chimeric H2A variant regulates mitotic H3-S10 dephosphorylation. Genes Dev 26(6):615-29.
CommentsB2086 cells Transformed with V5-PP50 with the cycloheximide resistance cassette H4-rpL29-B upstream. Partial replacement . Cells are very sick, give yellow-green auto fluorescence with many vacuoles.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02539
Requires MTA?No
Academic cost $45