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Stock IDSD02531
Strain NameT146VHA #1
AKA
SpeciesT. thermophila
Genetic Descriptiongtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, HAc(::GTU1, T146V)]; pm-r, ?)
MAC Knockins/outsGTU1 with mutation T146V and c-terminus HA tag into the MTT1 locus, partial replacement.
MIC Knockins/outsNeo2 into GTU1 locus
Gene of InterestGTU1 (TTHERM_00079520)
Origin
PublicationsShang Y, Li B, Gorovsky MA. 2002. Tetrahymena thermophila contains a conventional gamma-tubulin that is differentially required for the maintenance of different microtubule-organizing centers. J Cell Biol 158(7):1195-206.

Shang Y, Tsao CC, Gorovsky MA. 2005. Mutational analyses reveal a novel function of the nucleotide-binding domain of gamma-tubulin in the regulation of basal body biogenesis. J Cell Biol 171(6):1035-44.
CommentspT146V-HA rescued GTUKO 5x6 progeny having mutation @ GTU1 T146V gene, driven by MTT1 promoter. Needs Cd to induce. Has HA tag at c-terminus. Single cell (a)
These are same as previous ones, but new transformants, (one week old) not single celled and haven’t tested genotype yet

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02531
Requires MTA?No
Academic cost $35