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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.


Stock IDSD02528
Strain NameS10E+neo, ∆∆, #2
AKA
SpeciesT. thermophila
Genetic Descriptionhht1[∆::neo2]/hht1[∆::neo2];hht3[∆::neo2]/hht3[∆::neo2]; hht2[3’neo2,S10E]/ hht2[3’neo2,S10E]; mpr1-1/mpr1-1 (hht1[∆::neo2]]; hht3[∆::neo2]; hht2[3’neo2,S10E]; pm-r, mp-r, ?)
MAC Knockins/outsNeo KO of HHT1.HHT3,HHT2 has S10E mutaion and neo2 in 3’ flanking region
MIC Knockins/outsNeo KO of HHT1.HHT3,HHT2 has S10E mutaion and neo2 in 3’ flanking region
Gene of InterestHHT1 (TTHERM_00570560) + HHT2 (TTHERM_00189180)
Origin
PublicationsLiu Y, Song X, Gorovsky MA, Karrer KM. 2005. Elimination of foreign DNA during somatic differentiation in tetrahymena thermophila shows position effect and is dosage dependent. Eukaryot Cell 4(2):421-31.

Liu Y. 2003. Genetic studies of RNA-mediated DNA elimination in the developing macronuclei of tetrahymena thermophila. University of Rochester.

Cui B, Liu Y, Gorovsky MA. 2006. Deposition and function of histone H3 variants in tetrahymena thermophila. Mol Cell Biol 26(20):7719-30.
CommentsH3 with S10E mutation in a background with no wildtype H3 present.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02528
Requires MTA?No
Academic cost $45