Tetrahymena Stock Center| Stock ID | SD02507 |
| Strain Name | R-Tcd2-Cu428 |
| AKA | |
| Species | T. thermophila |
| Genetic Description | mpr1-1/mpr1-1 (tcd2[∆?::neo4]/TCD2[5’bsr1]; Think it is a mix of pm-r and bs-r. MPR1-1, VII) |
| MAC Knockins/outs | TCD2 was knocked out with neo4, then the wt TCD2 gene with a bsr1cassette upstream was somatically reintroduced into the cell |
| MIC Knockins/outs | |
| Gene of Interest | HPL7 (TTHERM_00551070) |
| Origin | |
| Publications | |
| Comments | Using Complete somatic Neo4 knockout Tcd2 strains. Tcd2 gene was reintroduced with blastcidin cassette (H4-BSR-B) upstream. H3K27 methylation was recovered. Bsr/Bsr, Tcd2/Tcd2, Neo4/Neo4 This is what Wei wrote, not sure if the replacement was complete or if both resistance are still present. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. |
| Research Resource Identifier | TSC_SD02507 |
| Requires MTA? | No |
| Academic cost | $45 |
