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Stock IDSD02507
Strain NameR-Tcd2-Cu428
AKA
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (tcd2[∆?::neo4]/TCD2[5’bsr1]; Think it is a mix of pm-r and bs-r. MPR1-1, VII)
MAC Knockins/outsTCD2 was knocked out with neo4, then the wt TCD2 gene with a bsr1cassette upstream was somatically reintroduced into the cell
MIC Knockins/outs
Gene of InterestHPL7 (TTHERM_00551070)
Origin
Publications
CommentsUsing Complete somatic Neo4 knockout Tcd2 strains. Tcd2 gene was reintroduced with blastcidin cassette (H4-BSR-B) upstream.
H3K27 methylation was recovered.

Bsr/Bsr, Tcd2/Tcd2, Neo4/Neo4 This is what Wei wrote, not sure if the replacement was complete or if both resistance are still present.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02507
Requires MTA?No
Academic cost $45