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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02506
Strain NameR-Tcd2-B2086
SpeciesT. thermophila
Genetic Description(tcd2[∆?::neo4]/TCD2[5’bsr1]; Mix of pm-r and bs-r, II)
MAC Knockins/outsTCD2 was knocked out with neo4, then the wt TCD2 gene with a bsr1cassette upstream was somatically reintroduced into the cell
MIC Knockins/outs
Gene of InterestHPL7 (TTHERM_00551070)
CommentsUsing Complete somatic Neo4 knockout Tcd2 strains. Tcd2 gene was reintroduced with blasticidin cassette (H4-BSR-B) upstream.
H3K27 methylation was recovered.

Bsr/Bsr, Tcd2/Tcd2, Neo4/Neo4 This is what Wei wrote, not sure if the replacement was complete or if both resistance are still present.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02506
Requires MTA?No
Academic cost $45