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Stock IDSD02504
Strain NameQ5A5
AKA
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (hho1[3’neo2, (T35A,S43A,S45A,T47A, T54A, K33Q,K38Q,K40Q,K50Q,K51Q)]; pm-r, VII)
MAC Knockins/outsChange Threonine 35,47,54 and serine 43,45 to alanine (charge patch) and 5 other sites to glutamine.
MIC Knockins/outs
Gene of InterestHHO1 (TTHERM_00823720)
Origin
PublicationsDou Y and Gorovsky MA. 2000. Phosphorylation of linker histone H1 regulates gene expression in vivo by creating a charge patch. Mol Cell 6(2):225-31.

Dou Y and Gorovsky MA. 2002. Regulation of transcription by H1 phosphorylation in tetrahymena is position independent and requires clustered sites. Proc Natl Acad Sci U S A 99(9):6142-6.
CommentsComplete somatic tranformant of CU428 cells with pQ5A5 /neo construct. Phosphorylation sites 1- 5 of H1 changed to alanines and 5 other sites changed to glutamines, neo 2 cassette in 3’ flanking region for selection. See paper.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02504
Requires MTA?No
Academic cost $35