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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02485
Strain NameMTT+E5
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (MTT1/mtt1[5’neo2, ∆,(::HHO1, T35E, S43E, S45E, T47E, T54E)]; pm-r, VII)
MAC Knockins/outsHHO1 with E5 charge patch inserted into coding region of MTT1
MIC Knockins/outs
Gene of InterestHHO1 (TTHERM_00823720)
PublicationsDou Y and Gorovsky MA. 2000. Phosphorylation of linker histone H1 regulates gene expression in vivo by creating a charge patch. Mol Cell 6(2):225-31.
CommentsMTT+E5 construct used for somatic transformaion of CU428, neo2 in 5’ flanking region

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02485
Requires MTA?No
Academic cost $45