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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02478
Strain NameK5E5-GFP
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (hho1[3’neo2, (T35E, S43E, S45E, T47E, T54E, T37K,A44K, T46K,V49K,V53K), GFPc]; pm-r, VII)
MAC Knockins/outsChange Threonine 35,47,54 and serine 43,45 to glutamic acid (charge patch) and T37K,A44K, T46K,V49K,V53K and GFP tag
MIC Knockins/outs
Gene of InterestHHO1 (TTHERM_00823720)
PublicationsDou Y and Gorovsky MA. 2000. Phosphorylation of linker histone H1 regulates gene expression in vivo by creating a charge patch. Mol Cell 6(2):225-31.

Dou Y and Gorovsky MA. 2002. Regulation of transcription by H1 phosphorylation in tetrahymena is position independent and requires clustered sites. Proc Natl Acad Sci U S A 99(9):6142-6.
CommentsComplete somatic tranformant of CU428 cells with pHHO5KE5 /neo construct. Phosphorylation sites 1- 5 of H1 changed to glutamic acid and 5 other sites changed to lysines,c-terminal GFP neo 2 cassette in 3’ flanking region for selection. See paper

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02478
Requires MTA?No
Academic cost $45