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Stock IDSD02477
Strain NameK5E5-3
AKA
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (hho1[3’neo2, T35E, S43E, S45E, T47E, T54E, P136K,IA137K, A141K,T145K, A148K]; pm-r, VII)
MAC Knockins/outsChange Threonine 35,47,54 and serine 43,45 to glutamic acid (charge patch) and P136K,IA137K, A141K,T145K, A148K
MIC Knockins/outs
Gene of InterestHHO1 (TTHERM_00823720)
Origin
PublicationsDou Y and Gorovsky MA. 2000. Phosphorylation of linker histone H1 regulates gene expression in vivo by creating a charge patch. Mol Cell 6(2):225-31.

Dou Y and Gorovsky MA. 2002. Regulation of transcription by H1 phosphorylation in tetrahymena is position independent and requires clustered sites. Proc Natl Acad Sci U S A 99(9):6142-6.
CommentsComplete somatic tranformant of CU428 cells with pHHO5K /neo construct. Phosphorylation sites 1- 5 of H1 changed to glutamic acid and 5 other sites changed to lysines, neo 2 cassette in 3’ flanking region for selection. See paper

From the Gorovsky lab, University of Rochester.
Research Resource IdentifierTSC_SD02477
Requires MTA?No
Academic cost $35