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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02463
Strain NameHTA3-T84G
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (HTAZ ; MTT1/mtt1[5’neo2,∆::HTAZ(K4R,K7R,K10R,K13R K16R,,K21R)]; pm-r, H2A.Z RRRRRRneo regulated by MTT1 promoter, VII)
MAC Knockins/outsinserted HTAZ with K4R,K7R,K10R,K13R K16R,,K21R Into MTT1 locus and neo2 in 5’flanking of MTT1
MIC Knockins/outs
Gene of InterestHTA3 (TTHERM_00143660)
PublicationsRen Q and Gorovsky MA. 2003. The nonessential H2A N-terminal tail can function as an essential charge patch on the H2A.Z variant N-terminal tail. Mol Cell Biol 23(8):2778-89.

Ren Q and Gorovsky MA. 2001. Histone H2A.Z acetylation modulates an essential charge patch. Mol Cell 7(6):1329-35.
CommentsCell has wt H2A.Z in its locus, and a mutated H2A.Z RRRRRR copy in MTT1 locus, neo2 cassette is inserted at 5’ of MTT, complete replacement ?. Somatic transformation.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02463
Requires MTA?No
Academic cost $45